Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1)

Weiss, C. A., Garnaat, C. W., Mukai, K., Hu, Y., Ma, H. (September 1994) Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1). Proc Natl Acad Sci U S A, 91 (20). pp. 9554-8. ISSN 0027-8424 (Print)

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We have isolated cDNAs from maize (ZGB1) and Arabidopsis (AGB1) encoding proteins homologous to beta subunits of guanine nucleotide-binding protein (G protein). The predicted ZGB1 and AGB1 gene products are 76% identical to each other and 41% or more identical to animal G protein beta subunits. Both predicted proteins contain seven repeats of the so-called "WD-40" motif, where WD is Trp-Asp. RNA blot analysis indicates that ZGB1 mRNA is present in the root, leaf, and tassel and that AGB1 mRNA is expressed in the root, leaf, and flower. DNA blot hybridizations indicate that maize and Arabidopsis genomes contain no other genes that are highly similar to ZGB1 and AGB1, respectively, suggesting that the newly isolated G protein beta-subunit homologues are likely to have unique functions. Furthermore, these G protein beta-subunit homologues are conserved among other plant species and may play important role(s) in plant signaling.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Arabidopsis/genetics/ metabolism Arabidopsis Proteins Base Sequence Comparative Study Conserved Sequence DNA, Complementary/isolation & purification GTP-Binding Protein beta Subunits GTP-Binding Proteins/ biosynthesis/genetics Gene Library Genes, Plant Heterotrimeric GTP-Binding Proteins Humans Macromolecular Substances Molecular Sequence Data Phylogeny Plant Proteins/ biosynthesis/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Restriction Mapping Sequence Homology, Amino Acid Zea mays/genetics/ metabolism
Subjects: organism description > plant > Arabidopsis
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 27 September 1994
Date Deposited: 06 Aug 2015 19:24
Last Modified: 06 Aug 2015 19:24
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