Druker, B. J., Neumann, M., Okuda, K., Franza, B. R., Griffin, J. D. (February 1994) Rel Is Rapidly Tyrosine-Phosphorylated Following Granulocyte-Colony-Stimulating Factor Treatment of Human Neutrophils. Journal of Biological Chemistry, 269 (7). pp. 5387-5390. ISSN 0021-9258
Abstract
Stimulation of neutrophils with granulocyte-colony stimulating factor (G-CSF) results in an enhanced respiratory burst, prolonged survival, and increased tumor cell killing. The effects of G-CSF are mediated by binding to specific, high affinity receptors. G-CSF receptors lack intrinsic tyrosine kinase activity, but activation of the receptor results in the rapid induction of tyrosine kinase activity. Antiphosphotyrosine immunoblots of whole cell lysates prepared from neutrophils show that the G-CSF rapidly induces prominent tyrosine phos phosphorylation of a protein of a relative molecular mass of 80 kDa. Using monospecific antibodies, the 80-kDa tyrosine-phosphorylated protein has been shown to be p80(c-rel), a proto-oncogene belonging to a family of transcriptional regulators which include NF-kB. The induction of tyrosine phosphorylation of p80(c-rel) was unique to G-CSF in that granulocyte-macrophage colony stimulating factor which also stimulates neutrophils and induces tyrosine phosphorylation does not result in tyro sine phosphorylation of p80(c-rel). The consequences of p80(c-rel) tyrosine phosphorylation are not yet known; however, tyrosine-phosphorylated p80(c-rel) is capable of binding to DNA, and G-CSF stimulation results in an increase in the amount of p80(c-rel) which binds to DNA, These results demonstrate that one of the first biochemical events which occurs in neutrophils following G-CSF stimulation, activation of a tyrosine kinase, leads directly to the tyrosine phosphorylation of p80(c-rel). Thus, the tyrosine kinase activated by G-CSF appears to directly transduce a signal to a protein which functions as a transcriptional regulator.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B DNA-BINDING SUBUNIT VIRUS STRAIN-T C-REL SIGNAL TRANSDUCTION FACTOR RECEPTOR PROTO-ONCOGENE PROTEINS DORSAL DROSOPHILA |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | February 1994 |
| Date Deposited: | 27 Aug 2015 19:34 |
| Last Modified: | 27 Aug 2015 19:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31392 |
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