Marinov, Georgi K, Wang, Jie, Handler, Dominik, Wold, Barbara J, Weng, Zhiping, Hannon, Gregory J, Aravin, Alexei A, Zamore, Phillip D, Brennecke, Julius, Toth, Katalin Fejes (March 2015) Pitfalls of Mapping High-Throughput Sequencing Data to Repetitive Sequences: Piwi’s Genomic Targets Still Not Identified. Developmental Cell, 32 (6). pp. 765-771. ISSN 1534-5807
Abstract
Summary Huang et al. (2013) recently reported that chromatin immunoprecipitation sequencing (ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Drosophila. Their study also reported that loss of Piwi causes widespread rewiring of transcriptional patterns, as evidenced by changes in RNA polymerase II occupancy across the genome. Here we reanalyze their data and report that the underlying deep-sequencing dataset does not support the authors’ genome-wide conclusions.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins Investigative techniques and equipment > Chromatin immunoprecipitation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Cancer Genetics CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 23 March 2015 |
| Date Deposited: | 25 Mar 2015 15:06 |
| Last Modified: | 15 Jul 2021 20:09 |
| PMCID: | PMC4494788 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31289 |
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