Meacham, C. E., Lawton, L. N., Soto-Feliciano, Y. M., Pritchard, J. R., Joughin, B. A., Ehrenberger, T., Fenouille, N., Zuber, J., Williams, R. T., Young, R. A., Hemann, M. T. (March 2015) A genome-scale in vivo loss-of-function screen identifies Phf6 as a lineage-specific regulator of leukemia cell growth. Genes & Development, 29 (5). pp. 483-8. ISSN 0890-9369
Abstract
We performed a genome-scale shRNA screen for modulators of B-cell leukemia progression in vivo. Results from this work revealed dramatic distinctions between the relative effects of shRNAs on the growth of tumor cells in culture versus in their native microenvironment. Specifically, we identified many "context-specific" regulators of leukemia development. These included the gene encoding the zinc finger protein Phf6. While inactivating mutations in PHF6 are commonly observed in human myeloid and T-cell malignancies, we found that Phf6 suppression in B-cell malignancies impairs tumor progression. Thus, Phf6 is a "lineage-specific" cancer gene that plays opposing roles in developmentally distinct hematopoietic malignancies.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes diseases & disorders > cancer > cancer types > leukemia |
| CSHL Authors: | |
| Communities: | CSHL labs > Lowe lab |
| Depositing User: | Matt Covey |
| Date: | 1 March 2015 |
| Date Deposited: | 13 Mar 2015 19:44 |
| Last Modified: | 15 Jul 2021 20:10 |
| PMCID: | PMC4358400 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31270 |
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