Yu, S., Yehia, G., Wang, J., Stypulkowski, E., Sakamori, R., Jiang, P., Hernandez-Enriquez, E. B., Tran, T. S., Bonder, E. M., Guo, W., Gao, N. (September 2014) Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion. Journal of Biological Chemistry, 289 (46). pp. 32030-32043. ISSN 0021-9258
Abstract
Rab11a has been conceived as a prominent regulatory component of the recycling endosome, which acts as a nexus in the endo- and exo-cytotic networks. The precise in vivo role of Rab11a in mouse embryonic development is unknown. We globally ablated Rab11a and examined the phenotypic and molecular outcomes in Rab11a-null blastocysts and mouse embryonic fibroblasts (MEFs). Using multiple trafficking assays and complementation analyses, we determined, among multiple important membrane-associated and soluble cargos, the critical contribution of Rab11a vesicular traffic to the secretion of multiple soluble MMPs. Rab11a-null embryos were able to properly form normal blastocysts but died at peri-implantation stages. Our data suggested that Rab11a critically controls mouse blastocyst development and soluble MMP secretion.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing organism description > animal > developmental stage > embryo bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > matrix metalloproteinase organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Mills lab CSHL Cancer Center Shared Resources > Gene Targeting Service |
| Depositing User: | Matt Covey |
| Date: | 30 September 2014 |
| Date Deposited: | 09 Oct 2014 19:06 |
| Last Modified: | 01 Dec 2015 21:26 |
| PMCID: | PMC4231680 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30832 |
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