Ley, S. C., Verbi, W., Pappin, D. J., Druker, B., Davies, A. A., Crumpton, M. J. (January 1994) Tyrosine phosphorylation of alpha tubulin in human T lymphocytes. Eur J Immunol, 24 (1). pp. 99-106. ISSN 0014-2980 (Print)0014-2980 (Linking)
Abstract
N-terminal sequencing of the 55- and 50-kDa polypeptides affinity purified on a phosphotyrosine monoclonal antibody column from activated Jurkat T cells identified alpha and beta tubulin. Two-dimensional gel analysis indicated that alpha tubulin was directly phosphorylated on tyrosine. beta Tubulin was not detectably tyrosine phosphorylated but was precipitated by anti-phosphotyrosine (PTyr) antibody by virtue of its association with the alpha subunit as a heterodimer. Phosphotyrosyl alpha tubulin was not incorporated into intact microtubules and was all in the unpolymerized soluble fraction. These results suggest that tyrosine phosphorylation of alpha tubulin may inhibit the ability of this subunit to polymerize into microtubules. Stimulation of Jurkat T cells via T cell receptor increased the amount of tubulin precipitated by the anti-PTyr antibody. These data raise the possibility that the polymerization of tubulin heterodimers may be regulated by phosphorylation on tyrosine during T cell activation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Electrophoresis, Gel, Two-Dimensional Humans Microtubules/ chemistry Molecular Sequence Data Phosphoproteins/ chemistry Phosphotyrosine Precipitin Tests T-Lymphocytes/ chemistry Tubulin/ chemistry Tumor Cells, Cultured Tyrosine/ analogs & derivatives/analysis |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | January 1994 |
| Date Deposited: | 09 Sep 2014 20:31 |
| Last Modified: | 09 Sep 2014 20:31 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30789 |
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