Koh-Luar, S. I., Parish, J. H., Bleasby, A. J., Pappin, D. J. C., Ainley, K., Johansen, F. E., McPherson, M. J., Radfors, A. (1989) Exported proteins of Neurospora crassa: 1-glucoamylase. Enzyme and Microbial Technology, 11 (10). pp. 692-695. ISSN 01410229 (ISSN)
Abstract
Polypeptide were exracted from the culture supernatants of Neurospora crassa growing in a variety of media. The polypeptides were analyzed by quantitative SDS-PAGE and the single polypeptide produced in the largest amount under the conditions tested was of molecular weight 69,000 and was referred to as p69. Synthesis of p69 was induced by starch and maltose, and studies on a partially purified preparation established that it was a glucoamylase. The sequence of the N-terminal 30 amino acids of p69 showed that this was related to sequence of analyses from Aspergillus spp. A synthetic oligonucleotide probe was synthesized and used to search for the corresponding gene(s) in Neurospora. A unique region was identified and mapped in a clone from a genomic library. The implications of the work for the development of Neurospora export and expression systems are discussed. © 1989.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Exported proteins fungal amylases Neurospora crassa |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organs, tissues, organelles, cell types and functions > tissues types and functions > transport > protein transport |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 1989 |
| Date Deposited: | 18 Sep 2014 19:49 |
| Last Modified: | 18 Sep 2014 19:49 |
| URI: | https://repository.cshl.edu/id/eprint/30752 |
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