Wilson, A. C., Parrish, J. E., Massa, H. F., Nelson, D. L., Trask, B. J., Herr, W. (January 1995) The gene encoding the VP16-accessory protein HCF (HCFC1) resides in human Xq28 and is highly expressed in fetal tissues and the adult kidney. Genomics, 25 (2). pp. 462-8. ISSN 0888-7543 (Print)
Abstract
After herpes simplex virus (HSV) infection, the viral regulatory protein VP16 activates transcription of the HSV immediate-early promoters by directing complex formation with two cellular proteins, the POU-homeodomain transcription factor Oct-1 and the host cell factor HCF. The function of HCF in uninfected cells is unknown. Here we show by fluorescence in situ hybridization and somatic cell hybrid analysis that the gene encoding human HCF, HCFC1, maps to the q28 region of the X chromosome. Yeast artificial chromosome and cosmid mapping localizes the HCFC1 gene within 100 kb distal of the renal vasopressin type-2 receptor (V2R) gene and adjacent to the renin-binding protein gene (RENBP). The HCFC1 gene is apparently unique. HCF transcripts and protein are most abundant in fetal and placental tissues and cell lines, suggesting a role in cell proliferation. In adults, HCF protein is abundant in the kidney, but not in the brain, a site of latent HSV infection and where HCF levels may influence progression of HSV infection.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence DNA, Complementary/genetics DNA-Binding Proteins/metabolism Fetal Proteins/biosynthesis/genetics Gene Expression Regulation Genes Herpes Simplex Virus Protein Vmw65/metabolism Host Cell Factor C1 Humans Hybrid Cells In Situ Hybridization, Fluorescence Kidney/ metabolism Molecular Sequence Data Octamer Transcription Factor-1 Organ Specificity Proteins/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Transcription Factors/metabolism X Chromosome |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > animal > developmental stage > fetal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > virus > herpes simplex virus organism description > animal > mammal > primates > hominids > human bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Jessica Koos |
| Date: | 20 January 1995 |
| Date Deposited: | 11 Aug 2014 20:40 |
| Last Modified: | 11 Aug 2014 20:40 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30650 |
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