Lahooti, H., White, R., Hoare, S. A., Rahman, D., Pappin, D. J., Parker, M. G. (December 1995) Identification of phosphorylation sites in the mouse oestrogen receptor. J Steroid Biochem Mol Biol, 55 (3-4). pp. 305-13. ISSN 0960-0760 (Print)0960-0760 (Linking)
Abstract
Phosphorylation sites in the mouse oestrogen receptor, expressed in COS-1 cells in the presence of 17 beta-oestradiol, have been mapped by solid phase microsequencing. The receptor was first radio-labelled with [32P]orthophosphate and a number of 3H- or 14C-labelled amino acids, immunopurified and then tryptic peptides were separated by thin layer chromatography or high performance liquid chromatography. Amino acid sequence analysis indicated that Ser-122, Ser-156, Ser-158 and Ser-298 were phosphorylated. The substitution of Ser-122 and Ser-298 with alanine had a negligible effect on the transcriptional activity of the receptor in transfected cells. However, a reduction of transcriptional activity was observed when Ser-122 was mutated in the context of mutations in a putative amphipathic alpha-helix involved in AF-2 activity. Thus a region of AF-1 that encompasses Ser-122 appears to interact with AF-2 in the full-length receptor.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 3T3 Cells Alanine Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line/metabolism Chromatography, High Pressure Liquid Estradiol/metabolism Mice Molecular Sequence Data Mutation Peptide Fragments/chemistry Phosphorylation Phosphoserine Receptors, Estrogen/chemistry/ genetics/ metabolism Sequence Analysis/methods Transcription, Genetic Transfection |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design organism description > animal > mammal > rodent > mouse bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Jessica Koos |
| Date: | December 1995 |
| Date Deposited: | 08 Aug 2014 20:40 |
| Last Modified: | 08 Aug 2014 20:40 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30612 |
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