Block, S. M., Svoboda, K. (April 1995) Analysis of high resolution recordings of motor movement. Biophys J, 68 (4 Supp). 230S-239S; discussion 239S. ISSN 0006-3495
Abstract
The development of in vitro motility assays for motor proteins has been accompanied by a parallel development of advanced optical instrumentation capable of recording motion at the molecular level. Devices now exist that can record displacements to better than 0.1 nm at bandwidths in excess of 10 kHz, and that can place controlled forces up to many pN on single motors. Ultra-high resolution data from experiments are now pouring in. The analysis and subsequent interpretation of experimental records, which are inevitably contaminated with high levels of thermal noise, remain an ongoing challenge. This essay examines selected issues relating to this challenge and discusses some alternative approaches.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Actins/physiology Adenosine Triphosphate/metabolism Animals Biophysics Humans Kinesin/physiology Microtubules/physiology Models, Biological Movement/physiology Muscle Contraction/physiology Myosins/physiology Proteins/chemistry/*physiology |
| Subjects: | physics > biophysics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin organism description > animal organism description > animal > mammal > primates > hominids > human organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Jessica Koos |
| Date: | April 1995 |
| Date Deposited: | 15 Aug 2014 16:30 |
| Last Modified: | 14 Nov 2019 13:39 |
| PMCID: | PMC1281932 |
| URI: | https://repository.cshl.edu/id/eprint/30569 |
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