Demonstration of adenosine receptors on mouse cerebral smooth muscle membranes

Beck, D. W., Vinters, H. V., Moore, S. A., Hart, M. N., Henn, F. A., Cancilla, P. A. (July 1984) Demonstration of adenosine receptors on mouse cerebral smooth muscle membranes. Stroke, 15 (4). pp. 725-7. ISSN 0039-2499 (Print)0039-2499

DOI: 10.1161/​01.STR.15.4.725


Adenosine receptors have been identified on brain cortical membranes and microvascular preparations. However, they have not been demonstrated on specific microvascular elements in isolation. 2-3H-chloroadenosine was used as a ligand to investigate the presence of adenosine receptors on isolated mouse cerebral smooth muscle membranes. The binding studies reveal the presence of a high affinity binding site with a Kd value of 33.3 nM and a maximal binding capacity (Bmax) of 283 fmol/mg protein. These findings demonstrate that there is an adenosine receptor on cerebral smooth muscle membranes.

Item Type: Paper
Uncontrolled Keywords: 2-Chloroadenosine Adenosine/analogs & derivatives/metabolism Animals Brain/*blood supply Cell Membrane/analysis Mice Microcirculation Muscle, Smooth, Vascular/*analysis Receptors, Cell Surface/*analysis/metabolism Receptors, Purinergic Tritium
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
organs, tissues, organelles, cell types and functions > tissues types and functions > smooth muscle
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: July 1984
Date Deposited: 24 Jun 2014 15:17
Last Modified: 24 Jun 2014 15:17
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