Beck, D. W., Vinters, H. V., Moore, S. A., Hart, M. N., Henn, F. A., Cancilla, P. A. (July 1984) Demonstration of adenosine receptors on mouse cerebral smooth muscle membranes. Stroke, 15 (4). pp. 725-7. ISSN 0039-2499 (Print)0039-2499
Abstract
Adenosine receptors have been identified on brain cortical membranes and microvascular preparations. However, they have not been demonstrated on specific microvascular elements in isolation. 2-3H-chloroadenosine was used as a ligand to investigate the presence of adenosine receptors on isolated mouse cerebral smooth muscle membranes. The binding studies reveal the presence of a high affinity binding site with a Kd value of 33.3 nM and a maximal binding capacity (Bmax) of 283 fmol/mg protein. These findings demonstrate that there is an adenosine receptor on cerebral smooth muscle membranes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 2-Chloroadenosine Adenosine/analogs & derivatives/metabolism Animals Brain/*blood supply Cell Membrane/analysis Mice Microcirculation Muscle, Smooth, Vascular/*analysis Receptors, Cell Surface/*analysis/metabolism Receptors, Purinergic Tritium |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor organs, tissues, organelles, cell types and functions > tissues types and functions > smooth muscle |
| CSHL Authors: | |
| Communities: | CSHL labs > Henn lab |
| Depositing User: | Matt Covey |
| Date: | July 1984 |
| Date Deposited: | 24 Jun 2014 15:17 |
| Last Modified: | 24 Jun 2014 15:17 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30230 |
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