Henn, F. A., Hansson, H. A., Hamberger, A. (June 1972) Preparation of plasma membrane from isolated neurons. Journal of Cell Biology, 53 (3). pp. 654-61. ISSN 0021-9525
Abstract
A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4-5-fold enrichment in plasma membrane markers, and a 10-15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphatases/analysis Animals Cell Fractionation *Cell Membrane/enzymology Cell Nucleolus Cell Nucleus Centrifugation, Density Gradient Cerebral Cortex/cytology/enzymology Cytochromes Electron Transport Complex IV/analysis Glucosephosphates/analysis Methods Microscopy, Electron Microsomes/enzymology Mitochondria/enzymology Monoamine Oxidase/analysis Nadp Neurons/*cytology/enzymology Oxidoreductases/analysis Rabbits Sucrose |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > membranes organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons |
| CSHL Authors: | |
| Communities: | CSHL labs > Henn lab |
| Depositing User: | Matt Covey |
| Date: | June 1972 |
| Date Deposited: | 09 Jul 2014 18:44 |
| Last Modified: | 09 Jul 2014 18:44 |
| PMCID: | PMC2108764 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30201 |
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