Demetrick, D. J., Zhang, H., Beach, D. H. (1996) Chromosomal mapping of the human genes CKS1 to 8q21 and CKS2 to 9q22. Cytogenet Cell Genet, 73 (3). pp. 250-4. ISSN 0301-0171 (Print)
Abstract
The human cdk2/cyclin A kinase complex is a key regulator of the events of S phase. This complex contains several proteins involved in regulating its catalytic activity, including one or more of the CKS proteins, which have recently been shown to inhibit the activation of the cdk2 kinase. To investigate whether the CKS genes may be altered in human neoplasia, we mapped the chromosome locations of CKS1 and CKS2 by fluorescence in situ hybridization (FISH). CKS1 was localized to 8q21, a locus that is seldom grossly altered in cancer. The localization of CKS2 to 9q22 places it very near to a putative tumour suppressor locus suggested to be responsible for susceptibility to the Basal Cell Nervus Syndrome (BCNS or Gorlin's syndrome) familial cancer disorder. Six fibroblast cell lines isolated from patients with BCNS were demonstrated by FISH to have both copies of CKS2 present. Partial sequencing of a genomic clone of CKS2 revealed that the open reading frame lies over three exons. Examination of the six cell lines by SSCP and PCR-based sequencing of the parts of the three exons coding for the full length protein demonstrated no consistent divergence from the reported cDNA sequence in any exon. It is unlikely that CKS2 is the BCNS tumour suppressor gene.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence CDC2-CDC28 Kinases Cell Line Chromosome Mapping Chromosomes, Human, Pair 8 Chromosomes, Human, Pair 9 Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases/ genetics DNA Primers Humans In Situ Hybridization, Fluorescence Molecular Sequence Data Protein-Serine-Threonine Kinases/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Matt Covey |
| Date: | 1996 |
| Date Deposited: | 22 May 2014 17:04 |
| Last Modified: | 22 May 2014 17:04 |
| URI: | https://repository.cshl.edu/id/eprint/30155 |
Actions (login required)
 |
Administrator's edit/view item |