A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM-1 is a ligand for CD11c/CD18

Blackford, J., Reid, H. W., Pappin, D. J., Bowers, F. S., Wilkinson, J. M. (March 1996) A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM-1 is a ligand for CD11c/CD18. Eur J Immunol, 26 (3). pp. 525-31. ISSN 0014-2980 (Print)0014-2980 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8605916
DOI: 10.1002/eji.1830260304


The rabbit CD11c molecule has been characterized by use of new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be cell surface event that is not dependent on intracellular signaling or on receptor cross-linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD18 and ICAM-1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM-1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM-1 do not aggregate. These observations suggest that ICAM-1 is a ligand for activated p150,95.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Antibodies, Monoclonal/ chemistry/pharmacology Antigen-Antibody Reactions Antigens, CD18/immunology Binding, Competitive/immunology Cations, Divalent Cell Aggregation/immunology Cell Line Immunophenotyping Integrin alphaXbeta2/ immunology/ metabolism Intercellular Adhesion Molecule-1/ immunology Ligands Male Mice Mice, Inbred BALB C Molecular Sequence Data Rabbits Signal Transduction/immunology T-Lymphocytes/ immunology Temperature
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > monoclonal antibody
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: March 1996
Date Deposited: 22 May 2014 19:42
Last Modified: 22 May 2014 19:42
Related URLs:
URI: https://repository.cshl.edu/id/eprint/30149

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