Blackford, J., Reid, H. W., Pappin, D. J., Bowers, F. S., Wilkinson, J. M. (March 1996) A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM-1 is a ligand for CD11c/CD18. Eur J Immunol, 26 (3). pp. 525-31. ISSN 0014-2980 (Print)0014-2980 (Linking)
Abstract
The rabbit CD11c molecule has been characterized by use of new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be cell surface event that is not dependent on intracellular signaling or on receptor cross-linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD18 and ICAM-1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM-1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM-1 do not aggregate. These observations suggest that ICAM-1 is a ligand for activated p150,95.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Antibodies, Monoclonal/ chemistry/pharmacology Antigen-Antibody Reactions Antigens, CD18/immunology Binding, Competitive/immunology Cations, Divalent Cell Aggregation/immunology Cell Line Immunophenotyping Integrin alphaXbeta2/ immunology/ metabolism Intercellular Adhesion Molecule-1/ immunology Ligands Male Mice Mice, Inbred BALB C Molecular Sequence Data Rabbits Signal Transduction/immunology T-Lymphocytes/ immunology Temperature |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > monoclonal antibody |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | March 1996 |
| Date Deposited: | 22 May 2014 19:42 |
| Last Modified: | 22 May 2014 19:42 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30149 |
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