Zhong, T., Luke, M. M., Arndt, K. T. (January 1996) Transcriptional regulation of the yeast DnaJ homologue SIS1. Journal of Biological Chemistry , 271 (3). pp. 1349-56. ISSN 0021-9258
Abstract
The Saccharomyces cerevisiae SIS1 gene encodes an essential heat shock protein with similarity to the Escherichia coli DnaJ protein. In sis 1-85 and sis1-86 mutants, the sis1 RNA is induced to high levels at room temperature and without heat shock. The presence of wild type SIS1 in the sis1-85 mutant represses the overexpression of SIS1-85 protein. Furthermore, overexpression of wild type SIS1 reduces the beta-galactosidase activity expressed from a SIS1:lacZ fusion. These results suggest that SIS1 negatively regulates its own expression. The autoregulation of SIS1 transcription is mediated through a 39-base pair cis-element containing the SIS1 heat shock element plus additional flanking sequences on one side. Although SIS1 transcription is constitutive, it is transiently induced upon heat shock. In addition, SIS1 transcription is regulated by SSA (a class of HSP70 proteins) function. The elevated transcription of SIS1 in ssa1 ssa2 mutants is mediated solely through the SIS1 heat shock element. Therefore, the SIS1 autoregulatory element is different from the SSA-responsive element, suggesting that the mechanism involved in autoregulation of SIS1 is distinct from regulation of SIS1 by SSA proteins.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Cloning, Molecular Comparative Study Escherichia coli/metabolism Escherichia coli Proteins Fungal Proteins/ biosynthesis/genetics Gene Expression Regulation, Fungal Genes, Fungal HSP40 Heat-Shock Proteins Heat Heat-Shock Proteins/biosynthesis/genetics Molecular Sequence Data Plasmids Promoter Regions (Genetics) RNA, Fungal/biosynthesis RNA, Messenger/biosynthesis Recombinant Fusion Proteins/biosynthesis Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ genetics/ metabolism Saccharomyces cerevisiae Proteins Transcription, Genetic beta-Galactosidase/biosynthesis |
| Subjects: | organism description > yeast > Saccharomyces Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Kathleen Darby |
| Date: | 19 January 1996 |
| Date Deposited: | 12 May 2014 17:21 |
| Last Modified: | 12 May 2014 17:21 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30128 |
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