Wu, G.-Y., Malinow, R., Cline, H. T.
(November 1996)
Maturation of a central glutamatergic synapse.
Science, 274 (5289).
pp. 972-6.
ISSN 0036-8075 (Print)
Abstract
Whole-cell recordings from optic tectal neurons in Xenopus tadpoles were used to study the maturation of a glutamatergic synapse. The first glutamatergic transmission is mediated only by N-methyl-D-aspartate (NMDA) receptors and is silent at resting potentials. More mature synapses acquire transmission by alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. This maturational program is mimicked by postsynaptic expression of constitutively active calcium-calmodulin-dependent protein kinase II (CaMKII). Newly formed synapses may be silent unless sufficient depolarization is provided by coincident activity that could activate postsynaptic CaMKII, resulting in the appearance of AMPA responses.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Ca(2+)-Calmodulin Dependent Protein Kinase/genetics/ metabolism
Evoked Potentials
Glutamates/ physiology
In Vitro
N-Methylaspartate/metabolism
Neurons/cytology/enzymology/physiology/virology
Patch-Clamp Techniques
Receptors, AMPA/ physiology
Receptors, N-Methyl-D-Aspartate/ physiology
Recombinant Proteins/metabolism
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Superior Colliculus/cytology/physiology
Synapses/ physiology
Synaptic Transmission
Xenopus
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal > Frog > xenopus |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Cline lab
CSHL labs > Malinow lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
8 November 1996 |
| Date Deposited: |
12 May 2014 17:05 |
| Last Modified: |
12 May 2014 17:05 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30125 |
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