Svoboda, K., Tank, D. W., Denk, W.
(May 1996)
Direct measurement of coupling between dendritic spines and shafts.
Science, 272 (5262).
pp. 716-9.
ISSN 0036-8075
Abstract
Characterization of the diffusional and electrotonic coupling of spines to the dendritic shaft is crucial to understanding neuronal integration and synaptic plasticity. Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons. Diffusional reequilibration was monitored with two-photon fluorescence imaging. The time course of reequilibration was exponential, with time constants in the range of 20 to 100 milliseconds, demonstrating chemical compartmentalization on such time scales. These values imply that electrical spine neck resistances are unlikely to exceed 150 megohms and more likely range from 4 to 50 megohms.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Dendrites/metabolism/*physiology/ultrastructure
Dextrans/metabolism
Diffusion
Electric Conductivity
Electric Impedance
Fluoresceins/metabolism
Fluorescence
Kinetics
Microscopy/methods
Models, Neurological
Neuronal Plasticity
Pyramidal Cells/metabolism/*physiology/ultrastructure
Rats |
| Subjects: |
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Svoboda lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
3 May 1996 |
| Date Deposited: |
13 May 2014 14:04 |
| Last Modified: |
13 May 2014 14:04 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30113 |
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