Fichtenbauer, D., Xu, X. M., Jackson, D., Kragler, F. (March 2012) The chaperonin CCT8 facilitates spread of tobamovirus infection. Plant Signaling & Behavior, 7 (3). pp. 318-21. ISSN 1559-2316
Abstract
The homeodomain transcription factor KNOTTED1 (KN1) functions in shoot meristem maintenance and is thought to move from cell to cell in a similar fashion as viral movement proteins. Both types of transported proteins bind to RNA, and associate with intercellular bridges formed by plasmodesmata. In a mutant screen for KN1 transport deficiency, a component of a type II chaperonin complex, CCT8, was identified, and found to interact with non-cell-autonomous proteins. The cct8 mutants are characterized by limited functionality of non-cell-autonomous proteins after their movement, and a phenotype resembling lack of homeodomain protein activity. Evidence suggests that CCT8 functions in post-translocational refolding of transported proteins. Here we show that spread of tobamovirus infection is reduced in a cct8 mutant. This suggests that similar to KN1, viral movement proteins are unfolded and refolded during transport to gain functionality in the receiving cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Chaperonins/genetics/*metabolism Meristem Plant Diseases/*virology Plant Proteins/genetics/*metabolism Tobamovirus/*pathogenicity |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > chaperonins organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| Depositing User: | Matt Covey |
| Date: | March 2012 |
| Date Deposited: | 12 May 2014 15:02 |
| Last Modified: | 12 May 2014 15:02 |
| PMCID: | PMC3443910 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30048 |
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