Shirke, A. M., Malinow, R.
(November 1997)
Mechanisms of potentiation by calcium-calmodulin kinase II of postsynaptic sensitivity in rat hippocampal CA1 neurons.
Journal of Neurophysiology, 78 (5).
pp. 2682-92.
Abstract
Mechanisms of potentiation by calcium-calmodulin kinase II of postsynaptic sensitivity in rat hippocampal CA1 neurons. J. Neurophysiol. 78: 2682-2692, 1997. Preactivated recombinant alpha-calcium-calmodulin dependent multifunctional protein kinase II (CaMKII*) was perfused internally into CA1 hippocampal slice neurons to test the effect on synaptic transmission and responses to exogenous application of glutamate analogues. After measurement of baseline transmission, internal perfusion of CaMKII* increased synaptic strength in rat hippocampal neurons and diminished the fraction of synaptic failures. After measurement of baseline responses to applied transmitter, CaMKII* perfusion potentiated responses to kainate but not responses to N-methyl--aspartate. Internal perfusion of CaMKII*potentiated the maximal effect of kainate. Potentiation by CaMKII* did not change the time course of responses to kainate, whereas increasing response size by pharmacologically manipulating desensitization or deactivation rate constants significantly altered the time course of responses. Nonstationary fluctuation analysis of responses to kainate showed a decrease in the coefficient of variation after potentiation by CaMKII*. These data support the hypothesis that CaMKII increases postsynaptic responsiveness by increasing the available number of active alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid/kainate channels and suggests that a similar process may occur during the expression of long-term potentiation.
| Item Type: |
Paper
|
| Additional Information: |
0022-3077 (Print)
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. |
| Uncontrolled Keywords: |
Animals
Artifacts
Ca(2+)-Calmodulin Dependent Protein Kinase/*pharmacology
Drug Synergism
Electric Stimulation
Hippocampus/*physiology
Kainic Acid/pharmacology
Models, Neurological
Perfusion
Pyramidal Cells/drug effects/*physiology
Rats
Rats, Sprague-Dawley
Reaction Time
Recombinant Proteins/pharmacology
Synapses/drug effects/physiology
Synaptic Transmission/drug effects/*physiology |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Malinow lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
November 1997 |
| Date Deposited: |
07 May 2014 17:24 |
| Last Modified: |
07 May 2014 17:24 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30020 |
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