Caligiuri, M., Connolly, T., Beach, D. (June 1997) Ran1 functions to control the Cdc10/Sct1 complex through Puc1. Molecular Biology of the Cell, 8 (6). pp. 1117-28. ISSN 1059-1524 (Print)
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Abstract
We have undertaken a biochemical analysis of the regulation of the G1/S-phase transition and commitment to the cell cycle in the fission yeast Schizosaccharomyces pombe. The execution of Start requires the activity of the Cdc2 protein kinase and the Sct1/Cdc10 transcription complex. Progression through G1 also requires the Ran1 protein kinase whose inactivation leads to activation of the meiotic pathway under conditions normally inhibitory to this process. We have found that in addition to Cdc2, Sct1/Cdc10 complex formation requires Ran1. We demonstrate that the Puc1 cyclin associates with Ran1 and Cdc10 in vivo and that the Ran1 protein kinase functions to control the association between Puc1 and Cdc10. In addition, we present evidence that the phosphorylation state of Cdc10 is altered upon inactivation of Ran1. These results provide biochemical evidence that demonstrate one mechanism by which the Ran1 protein kinase serves to control cell fate through Cdc10 and Puc1.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cell Cycle Cell Cycle Proteins/ physiology Cyclins/ physiology DNA-Binding Proteins/metabolism/ physiology Fungal Proteins/physiology Gene Expression Regulation, Fungal Peptide Mapping Phosphoserine/metabolism Protein Kinases/ physiology Protein-Serine-Threonine Kinases Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Schizosaccharomyces/ cytology/ genetics Schizosaccharomyces pombe Proteins Structure-Activity Relationship Transcription Factors/ physiology Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > yeast > Saccharomyces > Schizosaccharomyces pombe bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Kathleen Darby |
| Date: | June 1997 |
| Date Deposited: | 07 May 2014 20:06 |
| Last Modified: | 07 May 2014 20:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29997 |
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