Wu, G. Y., Cline, H. T.
(January 1998)
Stabilization of dendritic arbor structure in vivo by CaMKII.
Science, 279 (5348).
pp. 222-6.
ISSN 0036-8075 (Print)
Abstract
Calcium-calmodulin-dependent protein kinase II (CaMKII) promotes the maturation of retinotectal glutamatergic synapses in Xenopus. Whether CaMKII activity also controls morphological maturation of optic tectal neurons was tested using in vivo time-lapse imaging of single neurons over periods of up to 5 days. Dendritic arbor elaboration slows with maturation, in correlation with the onset of CaMKII expression. Elevating CaMKII activity in young neurons by viral expression of constitutively active CaMKII slowed dendritic growth to a rate comparable to that of mature neurons. CaMKII overexpression stabilized dendritic structure in more mature neurons, whereas CaMKII inhibition increased their dendritic growth. Thus, endogenous CaMKII activity limits dendritic growth and stabilizes dendrites, and it may act as an activity-dependent mediator of neuronal maturation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Axons/physiology/ultrastructure
Benzylamines/pharmacology
Ca(2+)-Calmodulin Dependent Protein Kinase/ metabolism
Carbocyanines
Dendrites/physiology/ ultrastructure
Fluorescent Dyes
Microscopy, Confocal
Neurons/cytology/ enzymology/physiology/ ultrastructure
Receptors, AMPA/metabolism
Receptors, N-Methyl-D-Aspartate/metabolism
Recombinant Proteins/metabolism
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Sulfonamides/pharmacology
Superior Colliculus/ cytology/enzymology
Synapses/metabolism
Synaptic Transmission
Time Factors
Vaccinia virus/genetics
Xenopus laevis |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal > Frog > xenopus |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Cline lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
9 January 1998 |
| Date Deposited: |
01 May 2014 13:53 |
| Last Modified: |
01 May 2014 13:53 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29941 |
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