Van Aelst, L.
(1998)
Two-hybrid analysis of Ras-Raf interactions.
Methods in Molecular Biology, 84.
pp. 201-22.
ISSN 1064-3745 (Print)
Abstract
The yeast two-hybrid system (1) is a genetic method that enables the experimentor to determine whether two proteins can form complexes within yeast cells. The method comprises expressing the proteins of interest as “hybrid” proteins, one fused to a DNA-binding domain and the other protein fused to a transcription-activating domain. If the fusion proteins interact, a reporter gene IS transcribed
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Genetic Techniques
Oncogene Proteins v-raf
Protein Binding
Retroviridae Proteins, Oncogenic/isolation & purification/ metabolism
Saccharomyces cerevisiae/genetics/metabolism
Transformation, Genetic
ras Proteins/isolation & purification/ metabolism |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Van Aelst lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
1998 |
| Date Deposited: |
01 May 2014 13:16 |
| Last Modified: |
01 May 2014 13:16 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29938 |
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