Liu, Y., Gong, W., Huang, C. C., Herr, W., Cheng, X. (July 1999) Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16. Genes and Development, 13 (13). pp. 1692-703. ISSN 0890-9369 (Print)
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Abstract
On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65; alphaTIF) forms a transcriptional regulatory complex-the VP16-induced complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive cis-regulatory elements in HSV immediate-early promoters called TAATGARAT. Comparison of different HSV VP16 sequences reveals a conserved core region that is sufficient for VP16-induced complex formation. The crystal structure of the VP16 core has been determined at 2.1 A resolution. The results reveal a novel, seat-like protein structure. Together with the activity of mutant VP16 proteins, the structure of free VP16 suggests that it contains (1) a disordered carboxy-terminal region that associates with HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured region involved in virion assembly and possessing a novel DNA-binding surface that differentiates among TAATGARAT VP16-response elements.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Comparative Study Crystallization Crystallography, X-Ray DNA/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Viral Herpes Simplex Virus Protein Vmw65/ chemistry/genetics/metabolism Herpesviridae/chemistry Host Cell Factor C1 Macromolecular Substances Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Octamer Transcription Factor-1 Protein Conformation Recombinant Fusion Proteins/genetics Regulatory Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Sequence Alignment Sequence Homology, Amino Acid Simplexvirus/ chemistry/physiology Trans-Activation (Genetics) Transcription Factors/metabolism |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organism description > virus > herpes simplex virus Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab CSHL labs > Xu lab |
| Depositing User: | Kathleen Darby |
| Date: | 1 July 1999 |
| Date Deposited: | 28 Apr 2014 13:34 |
| Last Modified: | 30 Apr 2014 17:33 |
| PMCID: | PMC316849 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29828 |
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