Svoboda, K., Helmchen, F., Denk, W., Tank, D. W. (January 1999) Spread of dendritic excitation in layer 2/3 pyramidal neurons in rat barrel cortex in vivo. Nature Neuroscience, 2 (1). pp. 65-73. ISSN 1097-6256 (Print)
Abstract
In layer 2/3 pyramidal neurons of barrel cortex in vivo, calcium ion concentration ([Ca2+]) transients in apical dendrites evoked by sodium action potentials are limited to regions close to the soma. To study the mechanisms underlying this restricted pattern of calcium influx, we combined two-photon imaging of dendritic [Ca2+] dynamics with dendritic membrane potential measurements. We found that sodium action potentials attenuated and broadened rapidly with distance from the soma. However, dendrites of layer 2/3 cells were electrically excitable, and direct current injections could evoke large [Ca2+] transients. The restricted pattern of dendritic [Ca2+] transients is therefore due to a failure of sodium action-potential propagation into dendrites. Also, stimulating subcortical activating systems by tail pinch can enhance dendritic [Ca2+] influx induced by a sensory stimulus by increasing cellular excitability, consistent with the importance of these systems in plasticity and learning.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Action Potentials/physiology Animals Calcium/metabolism Dendrites/metabolism/ physiology Electric Stimulation Membrane Potentials/physiology Microscopy, Confocal Osmolar Concentration Pain/metabolism/physiopathology Photons Physical Stimulation Pyramidal Cells/metabolism/ physiology Rats Rats, Inbred F344 Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Sodium/physiology Somatosensory Cortex/ physiology Vibrissae/ physiology |
| Subjects: | Investigative techniques and equipment organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Svoboda lab |
| Depositing User: | Kathleen Darby |
| Date: | January 1999 |
| Date Deposited: | 30 Apr 2014 14:41 |
| Last Modified: | 30 Apr 2014 14:41 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29807 |
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