Nusbaum, C., Slonim, D. K., Harris, K. L., Birren, B. W., Steen, R. G., Stein, L. D., Miller, J., Dietrich, W. F., Nahf, R., Wang, V., Merport, O., Castle, A. B., Husain, Z., Farino, G., Gray, D., Anderson, M. O., Devine, R., Horton, L. T., Ye, W., Wu, X., Kouyoumjian, V., Zemsteva, I. S., Wu, Y., Collymore, A. J., Courtney, D. F., Tam, J., Cadman, M., Haynes, A. R., Heuston, C., Marsland, T., Southwell, A., Trickett, P., Strivens, M. A., Ross, M. T., Makalowski, W., Xu, Y., Boguski, M. S., Carter, N. P., Denny, P., Brown, S. D., Hudson, T. J., Lander, E. S. (August 1999) A YAC-based physical map of the mouse genome. Nature Genetics, 22 (4). pp. 388-93.
Abstract
A physical map of the mouse genome is an essential tool for both positional cloning and genomic sequencing in this key model system for biomedical research. Indeed, the construction of a mouse physical map with markers spaced at an average interval of 300 kb is one of the stated goals of the Human Genome Project. Here we report the results of a project at the Whitehead Institute/MIT Center for Genome Research to construct such a physical map of the mouse. We built the map by screening sequenced-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content information with a dense genetic map. The integrated map shows the location of 9,787 loci, providing landmarks with an average spacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome. We also report the results of a project at the MRC UK Mouse Genome Centre targeted at chromosome X. The project produced a YAC-based map containing 619 loci (with 121 loci in common with the Whitehead map and 498 additional loci), providing especially dense coverage of this sex chromosome. The YAC-based physical map directly facilitates positional cloning of mouse mutations by providing ready access to most of the genome. More generally, use of this map in addition to a newly constructed radiation hybrid (RH) map provides a comprehensive framework for mouse genomic studies.
| Item Type: | Paper |
|---|---|
| Additional Information: | 1061-4036 (Print) Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
| Uncontrolled Keywords: | Animals Chromosome Mapping *Chromosomes, Artificial, Yeast Contig Mapping Genetic Markers *Genome Mice/*genetics Models, Genetic *Physical Chromosome Mapping |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Stein lab |
| Depositing User: | Kathleen Darby |
| Date: | August 1999 |
| Date Deposited: | 30 Apr 2014 15:00 |
| Last Modified: | 30 Apr 2014 15:00 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29786 |
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