Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant

Powers, S., Gonzales, E., Christensen, T., Cubert, J., Broek, D. (June 1991) Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell, 65 (7). pp. 1225-31. ISSN 0092-8674 (Print)0092-8674

Abstract

By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/genetics GTP-Binding Proteins/physiology Gene Expression Regulation, Fungal *Genes, Fungal Genes, Suppressor Molecular Sequence Data Multigene Family Restriction Mapping Saccharomyces cerevisiae/cytology/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Powers lab
Depositing User: Matt Covey
Date: 28 June 1991
Date Deposited: 26 Feb 2014 15:44
Last Modified: 26 Feb 2014 15:44
Related URLs:
URI: https://repository.cshl.edu/id/eprint/29518

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