Villanueva, A., Lozano, J., Morales, A., Lin, X. H., Deng, X. Z., Hengartner, M. O., Kolesnick, R. N. (September 2001) jkk-1 and mek-1 regulate body movement coordination and response to heavy metals through jnk-1 in Caenorhabditis elegans. Embo Journal, 20 (18). pp. 5114-5128. ISSN 0261-4189
Abstract
Although in vitro evidence suggests two c-Jun N-terminal kinase (JNK) kinases, MKK4 and MKK7, transactivate JNK, in vivo confirmation is incomplete. In fact, JNK deficiency may differ from the composite deficiency of MKK4 and MKK7 in Drosophila and mice. Recently, the Caenorhabditis elegans homolog of human JNK, jnk-1, and two MKK-7s, mek-1 and jkk-1, were cloned. Here we characterize jnk-1, which encodes two isoforms JNK-1 alpha and JNK-1 beta. A null allele,jnk-1(gk7), yielded worms with defective body movement coordination and modest mechanosensory deficits. Similarly to jkk-1 mutants, elimination of GABAergic signals suppressed the jnk-1(gk7) locomotion defect. Like mek-1 nulls, jnk-1(gk7) showed copper and cadmium hypersensitivity. Conditional expression of JNK-1 isoforms rescued these defects, suggesting that they are not due to developmental errors. While jkk-1 or mek-1 inactivation mimicked jnk-1(gk7) locomotion and heavy metal stress defects, respectively, mkk-4 inactivation did not, but rather yielded defective egg laying. Our results delineate at least two different JNK pathways through jkk-1 and mek-1 in C.elegans, and define interaction between MKK7, but not MKK4, and JNK.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | body movement Caenorhabditis elegans heavy metals jkk-1 mek-1 SIGNAL-TRANSDUCTION PATHWAY GLUTAMIC-ACID DECARBOXYLASE JUN NH2-TERMINAL KINASE DOUBLE-STRANDED-RNA PROTEIN-KINASE MAP KINASE C-ELEGANS GENETIC INTERFERENCE ACTIVATION DOMAIN INDUCED APOPTOSIS |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs > Hengartner lab |
| Depositing User: | Matt Covey |
| Date: | September 2001 |
| Date Deposited: | 16 Jan 2014 15:43 |
| Last Modified: | 16 Jan 2014 15:43 |
| PMCID: | PMC125628 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29322 |
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