Carl, S., Greenough, T. C., Krumbiegel, M., Greenberg, M., Skowronski, J., Sullivan, J. L., Kirchhoff, F. (April 2001) Modulation of different human immunodeficiency virus type 1 Nef functions during progression to AIDS. Journal of Virology, 75 (8). pp. 3657-3665. ISSN 0022-538X
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Abstract
The human immunodeficiency virus type 1 (HIV-1) Nef protein has several independent functions that might contribute to efficient viral replication in vivo. Since HIV-1 adapts rapidly to its host environment, we investigated if different Nef properties are associated with disease progression. Functional analysis revealed that nef alleles obtained during late stages of infection did not efficiently downmodulate class I major histocompatibility complex but were highly active in the stimulation of viral replication. In comparison, functional activity in downregulation of CD4 and enhancement of HIV-1 infectivity were maintained or enhanced after AIDS progression. Our results demonstrate that various Nef activities are modulated during the course of HIV-1 infection to maintain high viral loads at different stages of disease progression. These findings suggest that all in vitro Nef functions investigated contribute to AIDS pathogenesis and indicate that nef variants with increased pathogenicity emerge in a significant number of HIV-l-infected individuals.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CELL-SURFACE CD4 LONG-TERM SURVIVOR DOWN-REGULATION DISEASE PROGRESSION HIV-1 INFECTION EXPRESSION GENE LYMPHOCYTES COMPLEX REPLICATION |
| Subjects: | diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs > Skowronski lab |
| Depositing User: | Matt Covey |
| Date: | April 2001 |
| Date Deposited: | 22 Jan 2014 19:52 |
| Last Modified: | 22 Jan 2014 19:52 |
| PMCID: | PMC114857 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29223 |
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