Cytokinetic failure and asynchronous nuclear division in BHK cells overexpressing a truncated protein-tyrosine-phosphatase

Cool, D. E., Andreassen, P. R., Tonks, N. K., Krebs, E. G., Fischer, E. H., Margolis, R. L. (June 1992) Cytokinetic failure and asynchronous nuclear division in BHK cells overexpressing a truncated protein-tyrosine-phosphatase. Proceedings of the National Academy of Sciences of the United States of America, 89 (12). pp. 5422-5426. ISSN 0027-8424

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Abstract

Previous work has shown that a T-cell protein-tyrosine-phosphatase truncated in its carboxyl-terminal domain (DELTA-C11.PTP) has full enzymatic activity but no longer localizes in the particulate fraction of the cell. Two baby hamster kidney (BHK) cell lines overexpressing the truncated protein are markedly multinucleate, a state likely caused by a failure in cytokinesis. Nuclei within syncytial cells overexpressing DELTA-C11.PTP display a remarkable asynchronous entry into mitosis. The effects require tyrosine phosphatase activity because expression of an inactive form of the truncated enzyme yields cells indistinguishable from the parental cell line. Redistribution of the enzyme from the particulate to the soluble fraction is apparently important to these observed effects because cells overexpressing the full-length, wild-type enzyme are morphologically similar to controls. Further, when these cells contain more than one nucleus, their syncytial nuclei undergo mitosis synchronously.

Item Type:	Paper
Uncontrolled Keywords:	CELL CYCLE CYTOKINESIS SYNCYTIA MITOSIS DNA-REPLICATION FISSION YEAST CLEAVAGE FURROW KINASE DEPHOSPHORYLATION ACTIVATION MITOSIS CDC2 PHOSPHORYLATION CD45
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein tyrosine phosphatase
CSHL Authors:	Tonks, Nicholas K.
Communities:	CSHL labs > Tonks lab
Depositing User:	Matt Covey
Date:	June 1992
Date Deposited:	17 Dec 2013 15:16
Last Modified:	30 Sep 2019 14:42
PMCID:	PMC49304
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/29083

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