Regulation of distinct stages of skeletal muscle differentiation by mitogen-activated protein kinases

Bennett, A. M., Tonks, N. K. (November 1997) Regulation of distinct stages of skeletal muscle differentiation by mitogen-activated protein kinases. Science, 278 (5341). pp. 1288-1291. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/9360925

DOI: 10.1126/science.278.5341.1288

Abstract

The signal transduction pathway or pathways linking extracellular signals to myogenesis are poorly defined. Upon mitogen withdrawal from C2C12 myoblasts, the mitogen-activated protein kinase (MAPK) p42Erk2 is inactivated concomitant with up-regulation of muscle-specific genes, Overexpression of MAPK phosphatase-1 (MKP-1) inhibited p42Erk2 activity and was sufficient to relieve the inhibitory effects of mitogens on muscle-specific gene expression. Later during myogenesis, endogenous expression of MKP-1 decreased. MKP-1 overexpression during differentiation prevented myotube formation despite appropriate expression of myosin heavy chain, This indicates that muscle-specific gene expression is necessary but not sufficient to commit differentiated myocytes to myotubes and suggests a function for the MAPKs during the early and late stages of skeletal muscle differentiation.

Item Type:	Paper
Uncontrolled Keywords:	FIBROBLAST GROWTH-FACTOR CYCLIN D1 MYOGENIC DIFFERENTIATION MYOD FAMILY FACTOR-BETA EXPRESSION INHIBITION MYOBLASTS CELLS RAS
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Mitogen-activated protein kinase organs, tissues, organelles, cell types and functions > tissues types and functions > myoblast organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Tonks, Nicholas K. Bennett, Anton
Communities:	CSHL labs > Tonks lab
Depositing User:	Matt Covey
Date:	November 1997
Date Deposited:	18 Dec 2013 16:32
Last Modified:	18 Dec 2013 16:32
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/29047

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