Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

Dalton, J. E., Fear, J. M., Knott, S., Baker, B. S., McIntyre, L. M., Arbeitman, M. N. (September 2013) Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains. BMC Genomics, 14. ISSN 1471-2164 (Public Dataset)

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Abstract

Background: Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (Fru(M)). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. Results: By over-expressing individual Fru(M) isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three Fru(M) isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of Fru(M) isoforms, including many annotated with neuronal functions. By determining the binding sites of individual Fru(M) isoforms using SELEX we demonstrate that the distinct zinc finger domain of each Fru(M) isoforms confers different DNA binding specificities. A genome-wide search for these binding site sequences finds that the gene sets identified as induced by over-expression of Fru(M) isoforms in males are enriched for genes that contain the binding sites. An analysis of the chromosomal distribution of genes downstream of Fru(M) shows that those that are induced and repressed in males are highly enriched and depleted on the X chromosome, respectively. Conclusions: This study elucidates the different regulatory and DNA binding activities of three Fru(M) isoforms on a genome-wide scale and identifies genes regulated by these isoforms. These results add to our understanding of sex chromosome biology and further support the hypothesis that in some cell-types genes with male-biased expression are enriched on the X chromosome.

Item Type:	Paper
Uncontrolled Keywords:	Fruitless Sex hierarchy Drosophila Behavior Genomics RNA-seq male courtship behavior central-nervous-system determination gene fruitless drosophila-melanogaster neural circuitry expression patterns sexual orientation rna-seq doublesex dimorphism
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > zinc finger proteins
CSHL Authors:	Knott, Simon R.V.
Communities:	CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	September 2013
Date Deposited:	09 Dec 2013 17:54
Last Modified:	18 Mar 2015 13:35
PMCID:	PMC3852243
Related URLs:	Publisher
Dataset ID:	GSE50515
URI:	https://repository.cshl.edu/id/eprint/28884

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