Volpe, T. A., Kidner, C., Hall, I. M., Teng, G., Grewal, S. I., Martienssen, R. A.
(September 2002)
Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi.
Science, 297 (5588).
pp. 1833-7.
ISSN 1095-9203 (Electronic)0036-8075 (Linking)
Abstract
Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well as by modified histones, and influences both gene expression and chromosome segregation. In the fission yeast Schizosaccharomyces pombe, we deleted the argonaute, dicer, and RNA-dependent RNA polymerase gene homologs, which encode part of the machinery responsible for RNA interference (RNAi). Deletion results in the aberrant accumulation of complementary transcripts from centromeric heterochromatic repeats. This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function. We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Centromere/physiology
Chromosomal Proteins, Non-Histone/metabolism
DNA Methylation
DNA Transposable Elements
Endoribonucleases/genetics/metabolism
Gene Deletion
*Gene Silencing
Genes, Fungal
Heterochromatin/genetics/*metabolism
Histones/*metabolism
Lysine/*metabolism
Methylation
Models, Genetic
RNA Replicase/genetics/metabolism
RNA, Double-Stranded/metabolism
RNA, Fungal/metabolism
RNA, Small Interfering
RNA, Untranslated/genetics/*metabolism
Repetitive Sequences, Nucleic Acid
Ribonuclease III
Schizosaccharomyces/*genetics/metabolism
Schizosaccharomyces pombe Proteins/genetics/metabolism
Transcription, Genetic
Transgenes |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Grewal lab
CSHL labs > Martienssen lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
13 September 2002 |
| Date Deposited: |
28 Oct 2013 15:38 |
| Last Modified: |
19 Sep 2014 14:17 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28806 |
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