Uchiyama, K., Jokitalo, E., Kano, F., Murata, M., Zhang, X., Canas, B., Newman, R., Rabouille, C., Pappin, D., Freemont, P., Kondo, H. (December 2002) VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo. J Cell Biol, 159 (5). pp. 855-66. ISSN 0021-9525 (Print)0021-9525 (Linking)
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Abstract
NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating the v- and t-SNAREs, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP135 and p47 are shown to function in Golgi and ER assembly.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphatases/metabolism Amino Acid Sequence Animals Binding Sites Binding, Competitive Biological Markers/analysis Carrier Proteins/chemistry/metabolism/ physiology Cell Cycle Proteins/chemistry/ physiology Cell Line Cells, Cultured Conserved Sequence Cricetinae Cytosol/enzymology/metabolism DEAD-box RNA Helicases Endopeptidases Endoplasmic Reticulum/ physiology Golgi Apparatus/ physiology Hydrolysis Liver/cytology Membrane Fusion Membrane Proteins/chemistry/metabolism/ultrastructure Models, Biological Nuclear Proteins/chemistry/ physiology Peptides/chemistry Protein Binding Protein Conformation Rats Sequence Deletion Sequence Homology, Amino Acid |
| Subjects: | bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > ATPase organs, tissues, organelles, cell types and functions > organelles, types and functions > endoplasmic reticulum organs, tissues, organelles, cell types and functions > organelles, types and functions > golgi organs, tissues, organelles, cell types and functions > organelles, types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 9 December 2002 |
| Date Deposited: | 31 Oct 2013 14:28 |
| Last Modified: | 31 Oct 2013 14:29 |
| PMCID: | PMC2173386 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28801 |
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