Regulation of rho GTPases by crosstalk and neuronal activity in vivo

Li, Z., Aizenman, C. D., Cline, H. T. (February 2002) Regulation of rho GTPases by crosstalk and neuronal activity in vivo. Neuron, 33 (5). pp. 741-750. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/11879651

DOI: 10.1016/S0896-6273(02)00621-9

Abstract

Proper development of neurons depends on synaptic activity, but the mechanisms of activity-dependent neuronal growth are not well understood. The small GTPases, RhoA, Rac, and Cdc42, regulate neuronal morphogenesis by controlling the assembly and stability of the actin cytoskeleton. We report an in situ method to determine endogenous Rho GTPase activity in intact Xenopus brain. We use this method to provide evidence for crosstalk between Rho GTPases in optic tectal cells. Moreover, crosstalk between the Rho GTPases appears to affect dendritic arbor development in vivo. Finally, we demonstrate that optic nerve stimulation regulates Rho GTPase activity in a glutamate receptor-dependent manner. These data suggest a link between glutamate receptor function, Rho GTPase activity, and dendritic arbor growth in the intact animal.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTPase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Rho organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organism description > animal > Frog > xenopus
CSHL Authors:	Aizenman, Carlos D. Cline, Hollis T. Li, Zheng
Communities:	CSHL labs > Cline lab
Depositing User:	Matt Covey
Date:	February 2002
Date Deposited:	08 Jan 2014 15:44
Last Modified:	08 Jan 2014 15:44
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28735

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