Genetic organization of the Vibrio harveyi dnaA gene region and analysis of the function of the V-harveyi DnaA protein in Escherichia coli

Berenstein, D., Olesen, K., Speck, C., Skovgaard, O. (May 2002) Genetic organization of the Vibrio harveyi dnaA gene region and analysis of the function of the V-harveyi DnaA protein in Escherichia coli. Journal of Bacteriology, 184 (9). 2533-+. ISSN 0021-9193

URL: http://www.ncbi.nlm.nih.gov/pubmed/11948168

DOI: 10.1128/JB.184.9.2533-2538.2002

Abstract

The Vibrionaceae family is distantly related to Enterobacteriaceae within the group of bacteria possessing the Dam methylase system. We have cloned, sequenced, and analyzed the dnaA gene region of Vibrio harveyi and found that although the organization of the V. harveyi dnaA region differs from that of Escherichia coli, the expression of both genes is autoregulated and ATP-DnaA binds cooperatively to ATP-DnaA boxes in the dnaA promoter region. The DnaA proteins of V. harveyi and E. coli are interchangeable and function nearly identically in controlling dnaA transcription and the initiation of chromosomal DNA replication despite the evolutionary distance between these bacteria.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing organism description > bacteria bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
CSHL Authors:	Speck, Christian
Communities:	CSHL labs > Stillman lab
Depositing User:	Matt Covey
Date:	May 2002
Date Deposited:	09 Jan 2014 15:40
Last Modified:	09 Jan 2014 15:40
PMCID:	PMC134989
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28670

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