The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae

Livi, G. P., Hicks, J. B., Klar, A. J. S. (1990) The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae. Molecular and Cellular Biology, 10 (1). pp. 409-412. ISSN 02707306 (ISSN)

Abstract

The silent mating-type genes (HML and HMR) of Saccharomyces cerevisiae are kept under negative transcriptional control by the trans-acting products of the four MAR/SIR loci. MAR/SIR gene mutations result in the simultaneous derepression of HML and HMR gene expression. The sum1-1 mutation was previously identified as an extragenic suppressor of mutations in MAR1 (SIR2) and MAR2 (SIR3). As assayed genetically, sum1-1 is capable of restoring repression of silent mating-type information in cells containing mar1 or mar2 null mutations. We show here that the mating-type phenotype associated with sum1-1 results from a dramatic reduction in the steady-state level of HML and HMR gene transcripts. At the same time, the sum1-1 mutation has no significant effect on the level of each of the four MAR/SIR mRNAs.

Item Type: Paper
Uncontrolled Keywords: article dna transcription fungus fungus mutation mating nonhuman priority journal saccharomyces cerevisiae Blotting, Northern Gene Expression Regulation, Fungal Genes, Structural, Fungal RNA, Fungal RNA, Messenger Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Transcription, Genetic Fungi
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1990
Date Deposited: 09 Aug 2013 20:54
Last Modified: 12 Aug 2013 15:09
Related URLs:
URI: https://repository.cshl.edu/id/eprint/28520

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