Klar, A. J., McIndoo, J., Strathern, J. N., Hicks, J. B. (1980) Evidence for a physical interaction between the transposed and the substituted sequences during mating type gene transposition in yeast. Cell, 22 (1 I). pp. 291-298.
Abstract
Mating type switches in the yeast Saccharomyces cerevisiae occur by transposition of a replica of the 'source; unexpressed loci HML and HMR to the mating type locus (MAT). The incoming information replaces previously expressed DNA, resulting in an interconversion of MAT alleles. A strain of genotype HMLα/HMLα MATα/mata-missense HMRα/hmra-nonsense HO/ho generates cells with the genotype HMLα/HMLαMATα/MATaHMRα/hmra-nonsense HO/ho; that is, wild-type MATa + recombinants are produced efficiently by a strain in which the incoming a information and the resident mata allele bear different mutations. Production of the wild-type MATa recombinants requires the homothallism (switching) function, and the incoming a information and the resident mata allele must bear different mutations. This result is consistent with the formation of a heteroduplex between the incoming and the outgoing DNA at MAT. Thus a process of unidirectional gene conversion as a mechanism for mating type gene transposition is favored. A molecular model based on a single-strand transfer is proposed. Results also favor the idea that the direction of switching is controlled by cell's mating phenotype rather than by the genetic content of MAT.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | animal experiment fungus gene translocation heredity in vitro study mating saccharomyces cerevisiae Alleles Conjugation, Genetic DNA Transposable Elements Models, Genetic Mutation Recombination, Genetic Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1980 |
| Date Deposited: | 09 Aug 2013 20:58 |
| Last Modified: | 12 Aug 2013 13:38 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28482 |
Actions (login required)
 |
Administrator's edit/view item |