Copenhaver, G. P., Nickel, K., Kuromori, T., Benito, M. I., Kaul, S., Lin, X., Bevan, M., Murphy, G., Harris, B., Parnell, L. D., McCombie, W. R., Martienssen, R. A., Marra, M., Preuss, D.
(1999)
Genetic definition and sequence analysis of Arabidopsis centromeres.
Science, 286 (5449).
pp. 2468-2474.
ISSN 00368075 (ISSN)
Abstract
High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These regions exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180-base pair repeats. Unexpectedly, the DNA within the centromeres was not merely structural but also encoded several expressed genes. The regions flanking the centromeres were densely populated by repetitive elements yet experienced normal levels of recombination. The genetically defined centromeres were well conserved among Arabidopsis ecotypes but displayed limited sequence homology between different chromosomes, excluding repetitive DNA. This investigation provides a platform for dissecting the role of individual sequences in centromeres in higher eukaryotes.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Arabidopsis
article
centromere
gene mapping
genetic analysis
genetic recombination
nonhuman
priority journal
sequence analysis
Base Composition
Base Sequence
Conserved Sequence
Contig Mapping
Crosses, Genetic
Crossing Over, Genetic
DNA, Plant
Gene Expression
Genes, Plant
Meiosis
Models, Genetic
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
Retroelements
Sequence Analysis, DNA
Arabidopsis thaliana
Embryophyta
Eukaryota |
| Subjects: |
bioinformatics > genomics and proteomics > analysis and processing
organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organism description > plant |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Martienssen lab
CSHL labs > McCombie lab |
| Depositing User: |
Matt Covey
|
| Date: |
1999 |
| Date Deposited: |
25 Apr 2013 15:53 |
| Last Modified: |
25 Apr 2013 15:53 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28215 |
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