Sublette, E., Naik, M. U., Jiang, X., Osten, P., Valsamis, H., Osada, S., Ohno, S., Sacktor, T. C.
(1993)
Evidence for a new, high-molecular weight isoform of protein kinase C in rat hippocampus.
Neuroscience Letters, 159 (1-2).
pp. 175-178.
ISSN 03043940 (ISSN)
Abstract
We describe a new form of protein kinase C (PKC) with a molecular weight of 97 kDa, higher than the known forms of vertebrate PKC. This putative new high-molecular weight isoform, which we are calling PKC(HMW), is increased in the membrane fraction either upon application of phorbol esters or with afferent synaptic stimulation of Schaffer collaterals in hippocampal slices. The protein cross-reacts on immunoblot with affinity-purified polyclonal antiserum raised against a peptide derived from the carboxy-terminus of PKCη; it does not cross-react, however, with antiserum against the amino-terminal region of PKCη. In the tissues examined, PKC(HMW) is localized primarily in brain, in contrast to PKCη, which is found predominantly in lung and skin.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
brain
long-term potentiation
phorbol ester
PKC
PKCη
isoenzyme
protein kinase c
adolescent
animal tissue
article
brain slice
controlled study
hippocampus
immunoblotting
long term potentiation
nonhuman
priority journal
rat
Amino Acid Sequence
Animal
Isoenzymes
Molecular Sequence Data
Molecular Weight
Neurons, Afferent
Phorbol Esters
Rats
Support, U.S. Gov't, P.H.S. |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Osten lab |
| Depositing User: |
Matt Covey
|
| Date: |
1993 |
| Date Deposited: |
05 Apr 2013 02:25 |
| Last Modified: |
05 Apr 2013 02:25 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28091 |
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