Nitric oxide negatively regulates mammalian adult neurogenesis

Packer, M. A., Stasiv, Y., Benraiss, A., Chmielnicki, E., Grinberg, A., Westphal, H., Goldman, S. A., Enikolopov, G. (August 2003) Nitric oxide negatively regulates mammalian adult neurogenesis. Proceedings of the National Academy of Sciences of the United States of America, 100 (16). pp. 9566-9571. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/12886012

DOI: 10.1073/pnas.1633579100

Abstract

Neural progenitor cells are widespread throughout the adult central nervous system but only give rise to neurons in specific loci. Negative regulators of neurogenesis have therefore been postulated, but none have yet been identified as subserving a significant role in the adult brain. Here we report that nitric oxide (NO) acts as an important negative regulator of cell proliferation in the adult mammalian brain. We used two independent approaches to examine the function of NO in adult neurogenesis. In a pharmacological approach, we suppressed NO production in the rat brain by intraventricular infusion of an NO synthase inhibitor. In a genetic approach, we generated a null mutant neuronal NO synthase knockout mouse line by targeting the exon encoding active center of the enzyme. In both models, the number of new cells generated in neurogenic areas of the adult brain, the olfactory subependyma and the dentate gyrus, was strongly augmented, which indicates that division of neural stem cells in the adult brain is controlled by NO and suggests a strategy for enhancing neurogenesis in the adult central nervous system.

Item Type:	Paper
Uncontrolled Keywords:	NEURAL STEM-CELLS FOCAL CEREBRAL-ISCHEMIA RAT BRAIN NEUROTROPHIC FACTOR GROWTH-FACTOR NEURONAL DIFFERENTIATION DROSOPHILA DEVELOPMENT SUBVENTRICULAR ZONE PRECURSOR CELLS S-NITROSYLATION
Subjects:	therapies > stem cells > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult organism description > animal > developmental stage > adult organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal > developmental stage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons bioinformatics > genomics and proteomics > small molecules > nitric oxide organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat bioinformatics > genomics and proteomics > small molecules therapies > stem cells
CSHL Authors:	Enikolopov, Grigori N. Packer, Michael A. Stasiv, Yuri
Communities:	CSHL labs > Enikopolov lab
Depositing User:	Matt Covey
Date:	August 2003
Date Deposited:	02 Apr 2013 15:55
Last Modified:	10 Sep 2019 18:39
PMCID:	PMC170958
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27965

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