Takahashi, T., Svoboda, K., Malinow, R.
(March 2003)
Experience strengthening transmission by driving AMPA receptors into synapses.
Science, 299 (5612).
pp. 1585-1588.
ISSN 0036-8075
Abstract
The mechanisms underlying experience-dependent plasticity in the brain may depend on the AMPA subclass of glutamate receptors (AMPA-Rs). We examined the trafficking of AMPA-Rs into synapses in the developing rat barrel cortex. In vivo gene delivery was combined with in vitro recordings to show that experience drives recombinant GluR1, an AMPA-R subunit, into synapses formed between layer 4 and layer 2/3 neurons. Moreover, expression of the GluR1 cytoplasmic tail, a construct that inhibits synaptic delivery of endogenous AMPA-Rs during long-term potentiation, blocked experience-driven synaptic potentiation. In general, synaptic incorporation of AMPA-Rs in vivo conforms to rules identified in vitro and contributes to plasticity driven by natural stimuli in the mammalian brain.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
BARREL FIELD CORTEX
SYNAPTIC PLASTICITY
EXCITATORY SYNAPSES
HIPPOCAMPAL-NEURONS
IN-VIVO
TRAFFICKING
RAT
POTENTIATION
EXPRESSION
MECHANISM |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Malinow lab
CSHL labs > Svoboda lab |
| Depositing User: |
Matt Covey
|
| Date: |
March 2003 |
| Date Deposited: |
01 Jul 2013 18:43 |
| Last Modified: |
01 Jul 2013 18:43 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27898 |
Actions (login required)
 |
Administrator's edit/view item |
