Berg, L., Lusky, M., Stenlund, A., Botchan, M. R. (August 1986) Repression of bovine papilloma virus replication is mediated by a virally encoded trans-acting factor. Cell, 46 (5). pp. 753-62. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
Cells transformed with bovine papilloma virus type 1 mutants in the E6 or E6/7 genes are resistant to high-copy-number amplification of wild-type DNA after supertransfection. Transient and stable replication assays demonstrate this effect. If the supertransfected DNA has a mutation in a newly defined gene (M), this cellular immunity to high-copy-number replication is overcome, resulting in transient replication of the input DNA. In contrast, the resident plasmid does not participate in amplification and is maintained at a constant low copy number. Cotransformation of M- mutants and wild-type DNA into these cells leads to shutoff of replication of both genomes. Thus, M- mutants define a trans-acting negative modulator that regulates viral replication. This function is distinct from the positive factors required for replication. We propose a model that explains why the loss of E6 and E6/7 function leads to immunity of the infected cell.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Bovine papillomavirus 1/ genetics DNA Replication DNA, Viral/genetics Gene Amplification Genes, Viral Papillomaviridae/ genetics Plasmids Replicon Repressor Proteins/ physiology Transcription Factors/ physiology Viral Proteins/ physiology Virus Replication |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Matt Covey |
| Date: | 29 August 1986 |
| Date Deposited: | 12 Mar 2013 18:37 |
| Last Modified: | 12 Mar 2013 18:37 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27780 |
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