Stenlund, A., Moreno-Lopez, J., Ahola, H., Pettersson, U. (November 1983) European elk papillomavirus: characterization of the genome, induction of tumors in animals, and transformation in vitro. J Virol, 48 (2). pp. 370-6. ISSN 0022-538X (Print)0022-538X (Linking)
Abstract
The European elk papillomavirus (EEPV) genome was cloned in the BamHI cleavage site of the pBR322 vector. The cloned genome was used for construction of a physical map, employing restriction endonucleases BamHI, BglII, HindIII, PvuII, SacI, and XhoI. The sequence homology between the EEPV and bovine papillomavirus type 1 genomes was elucidated by performing hybridizations in different concentrations of formamide. Sequence homology could only be revealed under less stringent conditions, i.e., Tm - 43 degrees C. Nucleotide sequence information was also collected from the regions which lie adjacent to the three HindIII sites that are present in the EEPV genome. The results made it possible to align the EEPV and bovine papillomavirus type 1 genomes. Transformation by EEPV was demonstrated with the C127 mouse cell line, and fibrosarcomas were induced in young hamsters after subcutaneous injection. The transformed cells and the tumors contain multiple, nonintegrated copies of the EEPV genome. Virus particles could not be detected either in tumors or in transformed cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Bovine papillomavirus 1/genetics Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Cloning, Molecular Cricetinae DNA Restriction Enzymes Deer/microbiology Fibrosarcoma/ microbiology Genes, Viral Mesocricetus Mice Papillomaviridae/genetics/ physiology |
| Subjects: | diseases & disorders > cancer diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Matt Covey |
| Date: | November 1983 |
| Date Deposited: | 12 Mar 2013 18:56 |
| Last Modified: | 12 Mar 2013 18:56 |
| PMCID: | PMC255361 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27774 |
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