Inducing RNAi in C. elegans by Feeding with dsRNA-expressing E. coli

Hammell, C. M., Hannon, G. J. (December 2012) Inducing RNAi in C. elegans by Feeding with dsRNA-expressing E. coli. Cold Spring Harb Protoc, 2012 (12). ISSN 1559-6095 (Electronic)1559-6095 (Linking)

Abstract

Owing to the relative ease and high-throughput nature of ingestion-mediated RNAi, the feeding of engineered Escherichia coli to wild-type and mutant Caenorhabditis elegans has developed into the most productive and common method to probe the functions of C. elegans genes. This protocol includes two variations of RNAi by feeding: one in which L1 larvae are fed dsRNA-expressing E. coli in liquid culture (commonly used to score effects of RNAi on postembryonic larval development) and another involving feeding of late larval- or adult-staged animals on standard solid medium culture plates.

Item Type:	Paper
Subjects:	organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment Investigative techniques and equipment > RNAI bioinformatics > genomics and proteomics > design > nucleic acid design > RNAI Design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi organism description > animal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > dsRNA
CSHL Authors:	Hammell, Christopher M. Hannon, Gregory J.
Communities:	CSHL labs > Hammell C. lab CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	1 December 2012
Date Deposited:	11 Mar 2013 16:55
Last Modified:	11 Mar 2013 16:55
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27740

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