Zink, M., Otto, C., Zorner, B., Zacher, C., Schutz, G., Henn, F. A., Gass, P. (April 2004) Reduced expression of brain-derived neurotrophic factor in mice deficient for pituitary adenylate cyclase activating polypeptide type-I-receptor. Neurosci Lett, 360 (1-2). pp. 106-8. ISSN 0304-3940 (Print)0304-3940 (Linking)
Abstract
In vitro pituitary adenylate cyclase activating polypeptide (PACAP) induces the expression of brain-derived neurotrophic factor (BDNF) via its specific receptor PAC1. Since BDNF has been implicated in learning paradigms and mice lacking functional PAC1 have deficits in hippocampus-dependent associative learning, we investigated whether PAC1 mutants show alterations in hippocampal expression of BDNF and its receptor TrkB. Semi-quantitative in situ-hybridization using exon-specific BDNF-probes revealed significantly reduced expression of the exon-III and exon-V-specific transcripts within the hippocampal CA3 region in PAC1-deficient mice. A similar trend was observed for the exon-I-specific transcript. The expression of the exon-III-specific transcript was also reduced within the dentate gyrus, while Trk B-expression did not differ between genotypes. Our data demonstrate that even in vivo PAC1-mediated signaling seems to play a pivotal role for the transcriptional regulation of BDNF.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Brain-Derived Neurotrophic Factor/ biosynthesis/genetics Gene Expression Regulation/physiology Hippocampus/metabolism Male Mice Mice, Inbred C57BL Mice, Transgenic Pilot Projects Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I Receptors, Pituitary Hormone/ deficiency/genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organism description > animal > mammal > rodent > mouse bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs > Henn lab |
| Depositing User: | Matt Covey |
| Date: | 22 April 2004 |
| Date Deposited: | 04 Mar 2013 21:34 |
| Last Modified: | 04 Mar 2013 21:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27701 |
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