Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2

Sartorius, A., Schloss, P., Vollmayr, B., Ende, G., Neumann-Haefelin, C., Hoehn, M., Henn, F. A. (2006) Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2. World J Biol Psychiatry, 7 (4). pp. 246-50. ISSN 1562-2975 (Print)1562-2975 (Linking)

Abstract

Hippocampal choline-containing compounds (Cho) determined with 1H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p = 0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.

Item Type:	Paper
Uncontrolled Keywords:	Animals Blotting, Western/methods Choline/ metabolism Electroconvulsive Therapy/ methods Hippocampus/ metabolism Magnetic Resonance Spectroscopy/ methods Male Phospholipases A/ metabolism Phospholipases A2 Rats Rats, Sprague-Dawley
Subjects:	Investigative techniques and equipment organism description > animal organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus Investigative techniques and equipment > spectroscopy > magnetic resonance spectroscopy organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat Investigative techniques and equipment > spectroscopy organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Henn, Fritz A.
Communities:	CSHL labs > Henn lab
Depositing User:	Matt Covey
Date:	2006
Date Deposited:	01 Mar 2013 17:22
Last Modified:	01 Mar 2013 17:22
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27657

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