Subcellular Dynamics of Type II PKA in Neurons

Zhong, H., Sia, G.-M., Sato, T. R., Gray, N. W., Mao, T., Khuchua, Z., Huganir, R. L., Svoboda, K. (May 2009) Subcellular Dynamics of Type II PKA in Neurons. Neuron, 62 (3). pp. 363-374.

URL: http://www.ncbi.nlm.nih.gov/pubmed/19447092

DOI: 10.1016/j.neuron.2009.03.013

Abstract

Summary Protein kinase A (PKA) plays multiple roles in neurons. The localization and specificity of PKA are largely controlled by A-kinase anchoring proteins (AKAPs). However, the dynamics of PKA in neurons and the roles of specific AKAPs are poorly understood. We imaged the distribution of type II PKA in hippocampal and cortical layer 2/3 pyramidal neurons in vitro and in vivo. PKA was concentrated in dendritic shafts compared to the soma, axons, and dendritic spines. This spatial distribution was imposed by the microtubule-binding protein MAP2, indicating that MAP2 is the dominant AKAP in neurons. Following cAMP elevation, catalytic subunits dissociated from the MAP2-tethered regulatory subunits and rapidly became enriched in nearby spines. The spatial gradient of type II PKA between dendritic shafts and spines was critical for the regulation of synaptic strength and long-term potentiation. Therefore, the localization and activity-dependent translocation of type II PKA are important determinants of PKA function.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Zhong, Haining
Communities:	CSHL labs > Svoboda lab
Depositing User:	Matt Covey
Date:	14 May 2009
Date Deposited:	19 Feb 2013 19:29
Last Modified:	19 Feb 2013 19:29
PMCID:	PMC2702487
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27464

Actions (login required)

Administrator's edit/view item