Nakano-Kobayashi, A., Nadif Kasri, N., Newey, S. E., Van Aelst, L. (July 2009) The Rho-Linked Mental Retardation Protein OPHN1 Controls Synaptic Vesicle Endocytosis via Endophilin A1. Curr Biol, 19 (13). 1133-1139 .
Abstract
Summary Neurons transmit information at chemical synapses by releasing neurotransmitters that are stored in synaptic vesicles (SVs) at the presynaptic site. After release, these vesicles need to be efficiently retrieved in order to maintain synaptic transmission [1], [2] and [3]. In concurrence, malfunctions in SV recycling have been associated with cognitive disorders [4] and [5]. Oligophrenin-1 (OPHN1) encodes a Rho-GTPase-activating protein (Rho-GAP) whose loss of function causes X-linked mental retardation [6] and [7]. OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons [8]. Previous studies report that postsynaptic OPHN1 is important for dendritic spine morphogenesis [8] and [9], but its function at the presynaptic site remains largely unexplored. Here, we present evidence that reduced or defective OPHN1 signaling impairs SV cycling at hippocampal synapses. In particular, we show that OPHN1 knockdown affects the kinetic efficiency of endocytosis. We further demonstrate that OPHN1 forms a complex with endophilin A1, a protein implicated in membrane curvature generation during SV endocytosis [10], [11], [12], [13], [14], [15] and [16] and, importantly, that OPHN1's interaction with endophilin A1 and its Rho-GAP activity are important for its function in SV endocytosis. Our findings suggest that defects in efficient SV retrieval may contribute to the pathogenesis of OPHN1-linked cognitive impairment.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CELLBIO MOLNEURO HUMDISEASE |
| Subjects: | diseases & disorders > congenital hereditary genetic diseases > mental retardation organs, tissues, organelles, cell types and functions organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: | |
| Communities: | CSHL labs > Van Aelst lab CSHL Cancer Center Shared Resources > Animal Services CSHL Cancer Center Shared Resources > DNA Sequencing Service CSHL Cancer Center Shared Resources > Instrumentation Service CSHL Cancer Center Shared Resources > Microscopy Service |
| Depositing User: | Matt Covey |
| Date: | 14 July 2009 |
| Date Deposited: | 20 Feb 2013 14:28 |
| Last Modified: | 30 Dec 2014 16:28 |
| PMCID: | PMC2711225 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27445 |
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